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. 2018 Jan 11;20(2):192–204. doi: 10.1177/1099800417750746

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Traditional Sanger sequencing compared with next-generation sequencing technology. In each method, dyed, unextendable bases are utilized to create a fluorescent signal that can be translated into a sequence of nucleotides. Subtle differences in the two methods lead to vast differences in throughput. This image was reproduced from figure 1 in Muzzey, Evans, and Lieber (2015). It is licensed under Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).