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. 2017 Dec 22;33(7):1129–1137. doi: 10.1093/ndt/gfx332

FIGURE 2.

FIGURE 2

Cardiac hypertrophy only develops in slow-progressing B6-Col4a3KO mice with advanced CKD. (A) M-mode echocardiography and representative cardiac midchamber histology in 10-week-old 129Sv, 10-week-old B6 and 20-week-old B6 WT and Col4a3KO mice. Bright-field microscopy: H&E (composite, scale bar  =  1  mm) staining showing cardiac morphology; fluorescence microscopy: WGA and merged WGA and DAPI staining (merge, scale bar  =  100  µm) showing cardiomyocyte size and number. (B) Heart weight:tibia length ratio. (C) Cardiomyocyte cross-sectional area calculated from WGA stained sections. (D) Cardiac mRNA expression of markers of hypertrophy (ANP, BNP and β-MHC). Values are mean  ±  standard error, n  =  4–7 per group. P  <  0.05 versus *WT, #all 129Sv and **all groups.