Fig. (1).

The effect of rs7959801T>C genotypes on MSI1 biological phenotypes. (A) Schematic diagram of the reporter constructs containing 2950 bp promoter of MSI1 with rs7959801T or C allele. (B) Luciferase activity of the MSI1 promoter constructs in two lung cancer cell lines (A549 and NCI-H520). The luciferase activity of each construct was normalised against the internal control of renilla luciferase. Columns, mean from three independent experiments; bars, standard deviation; Student’s t-test was used to compare the expression levels of different constructs. (C) mRNA expression of MSI1 in lung cancer tissues and adjacent normal lung tissues. (D) MSI1 mRNA expression in lung cancer tissues with rs7959801T>C genotypes. (E) Protein expressions in lung cancer tissues in situ using the Image Pro Plus software to score the MSI1 expression (magnification ×200). The comparisions of MSI1 expression in different genotypes were measured with one-way ANOVA test.