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. 2018 Apr 24;27(14):2443–2453. doi: 10.1093/hmg/ddy145

Figure 2.

Figure 2.

Global adult inducible Fig4 deletion results in severe motor defects, weight loss, and death. (A) The graph shows animal weight (g) as function of days post tamoxifen injection for Fig4 control and adult Fig4 iKO mice. Day 0 denotes the first tamoxifen injection when mice were between P60 and P90. Fig4 control mice, n = 10 (day 0) and n = 8 (day 60). For iKO mice, n = 5 (day 0) and n = 3 (day 60). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. 2-way ANOVA with Sidak correction. (B, C) Tail suspension test with Fig4 control and adult Fig4 iKO(60→90) mice. Control mice display typical hindlimb spreading, whereas iKO mice show abnormal clasping and truncal twisting. (D, E) Representative images of Fig4 control and adult Fig4 iKO(60→120) mice. iKO mice show spotty loss of fur pigmentation. (F) Quantification of tail suspension test. Animals were held by their tails for 15s and time displaying hindlimb splaying was recorded. Data presented as percentage of total time hindlimb splaying, mean ± SEM, ****P < 0.0001, unpaired Student t-test. n = 3 (control) and n = 5 (iKO).