Figure 2.

ExSpeU1s counteract the effect of inhibitory splicing factors. ELP1 mutant minigene was co-transfected with different ExSpeU1s (Ik4–72) along with plasmids coding for the indicated inhibitory splicing factors in Hek293T cells: hnRNP A1, FUS and SRSF3. The exon 20 inclusion and exon 20 skipping bands are indicated. The upper band of 349 bp corresponds to transcripts including exon 20; the lower band of 275 bp to exon 20 skipping. The PCR products were separated on a 2% agarose gel and the intensity of the bands were measured with ImageJ software. The percentage of exon 20 inclusion expressed as mean±SD of three independent experiments is indicated.