Figure 1.

Immunoblotting analysis and soluble Aβ levels in HT22 cells transfected with mutant APP cDNA. Figure 1A shows representative immunoblotting analysis of mutant APP-HT22 cells. Mouse primary hippocampal (HT22) cells were transfected with mutant APP cDNA (with Swe/Indiana mutations) for 48 h and cells were harvested and protein lysates were made and resolved on 4–20 gradient gel and probed with 6E10 antibody that recognizes full-length mutant APP. A-110 mutant full-length APP protein was found in mutant APP-HT22 cells, but not in untransfected HT22 cells. Figure 1B shows Aβ42 and 40 levels in HT22 cells transfected with mutant APP cDNA.