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Immunofluorescence analysis of synaptic and dendritic proteins in HT22 cells transfected with mutant APP cDNA relative to untransfected HT22 cells.Figure 7A presents representative images of immunofluorescence analysis. Figure 7B represents quantitative immunofluorescence analysis. Significantly increased levels of synaptophysin (P=0.002), PSD95 (P=0.001) and MAP2 (P=0.04) were found in HT22 cells transfected with mutant APP cDNA relative to untransfected HT22 cells.
