Table 2.
Summary of qRT-PCR oligonucleotide primers used in measuring mRNA expressions in mitochondrial dynamics and mitochondrial biogenesis, synaptic, autophagy and mitophagy genes in mAPP-HT22 cells compared to untransfected WT-HT22 cells
| Gene | DNA sequence (5′-3′) | PCR product size |
|---|---|---|
| Mitochondrial dynamics genes | ||
| Drp1 | Forward primer ATGCCAGCAAGTCCACAGAA | 86 |
| Reverse primer TGTTCTCGGGCAGACAGTTT | ||
| Fis1 | Forward primer CAAAGAGGAACAGCGGGACT | 95 |
| Reverse primer ACAGCCCTCGCACATACTTT | ||
| Mfn1 | Forward primer GCAGACAGCACATGGAGAGA | 83 |
| Reverse primer GATCCGATTCCGAGCTTCCG | ||
| Mfn2 | Forward primer TGCACCGCCATATAGAGGAAG | 78 |
| Reverse primer TCTGCAGTGAACTGGCAATG | ||
| Opa1 | Forward primer ACCTTGCCAGTTTAGCTCCC | 82 |
| Reverse primer TTGGGACCTGCAGTGAAGAA | ||
| Mitochondrial biogenesis genes | ||
| PGC1α | Forward primer GCAGTCGCAACATGCTCAAG | 83 |
| Reverse primer GGGAACCCTTGGGGTCATTT | ||
| Nrf1 | Forward primer AGAAACGGAAACGGCCTCAT | 96 |
| Reverse primer CATCCAACGTGGCTCTGAGT | ||
| Nrf2 | Forward primer ATGGAGCAAGTTTGGCAGGA | 96 |
| Reverse primer GCTGGGAACAGCGGTAGTAT | ||
| TFAM | Forward primer TCCACAGAACAGCTACCCAA | 84 |
| Reverse primer CCACAGGGCTGCAATTTTCC | ||
| Reverse primer AGACGGTTGTTGATTAGGCGT | ||
| Synaptic genes | ||
| Synaptophysin | Forward primer CTGCGTTAAAGGGGGCACTA | 81 |
| Reverse primer ACAGCCACGGTGACAAAGAA | ||
| PSD95 | Forward primer CTTCATCCTTGCTGGGGGTC | 90 |
| Reverse primer TTGCGGAGGTCAACACCATT | ||
| Autophagy genes | ||
| LC3A | Forward primer CCCATCGCTGACATCTATGAAC | 77 |
| Reverse primer AAGGTTTCTTGGGAGGCGTA | ||
| LC3B | Forward primer TCCACTCCCATCTCCGAAGT | 94 |
| Reverse primer TTGCTGTCCCGAATGTCTCC | ||
| ATG5 | Forward primer TCCATCCAAGGATGCGGTTG | 95 |
| Reverse primer TCTGCATTTCGTTGATCACTTGAC | ||
| Beclin1 | Forward primer ACCAGCGGGAGTATAGTGAGT | 98 |
| Reverse primer CAGCTGGATCTGGGCGTAG | ||
| Mitophagy genes | ||
| Pink1 | Forward primer CCATCGGGATCTCAAGTCCG | 70 |
| Reverse primer GATCACTAGCCAGGGACAGC | ||
| TERT | Forward primer GCAAGGTGGTGTCTGCTAGT | 100 |
| Reverse primer AGCTTGCCGTATTTCCCCAA | ||
| BCL2 | Forward primer TCCTTCCAGCCTGAGAGCAA | 73 |
| Reverse primer GCCTGAGAGGAGACGTCCTG | ||
| BNIP3L | Forward primer GCACGTTCCTTCCTCGTCT | 82 |
| Reverse primer GCTCTGTCCCGACTCATGC | ||
| Housekeeping genes | ||
| B-actin | Forward primer AGAAGCTGTGCTATGTTGCTCTA | 91 |
| Reverse primer TCAGGCAGCTCATAGCTCTTC | ||
| GAPDH | Forward primer TTCCCGTTCAGCTCTGGG | 59 |
| Reverse primer CCCTGCATCCACTGGTGC | ||