Figure 7.

Knockdown of spastin and AP5Z1 recapitulates neurite outgrowth defects. (A–C) qRT-PCR expression of the C-terminus of spastizin transcript (A), N-terminus of spastizin transcript (B), or C-terminus of AP5Z1 transcript (C). (D) Representative images of week 6 knockdown forebrain neurons following 48 h treatment with vehicle or 10 μM mdivi-1. Scale bars: 20 µm. (E) Quantification of axon length in week 6 neurons. (F) Primary neurite length in week 6 neurons. Data are presented as means ± SEM from a minimum of 30 cells in three independent experiments. *P < 0.05 versus Luc RNAi or vehicle-treated groups.