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. 2018 May 8;39(7):937–947. doi: 10.1093/carcin/bgy046

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© The Author(s) 2018. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — AFF4 promotes migration and invasion of HNSCC cells. (a, b) Wound-healing assay performed to assess the effects of AFF4 depletion on migration activity of SCC1 cells (a) and SCC23 cells (b). Bar indicates 400 μm. (c) Quantification of migration distance in a and b. n = 3. (d) Transwell assay conducted to study the effect of AFF4 depletion on invasion activity of SCC1 cells and SCC23 cells. Bar indicates 200 μm. n = 3. (e, f) Wound-healing assay performed to assess the effects of AFF4 overexpression on migration activity of SCC1 cells (e) and SCC23 cells (f). Bar indicates 400 μm. (g) Quantification of migration distance in e and f. n = 3. (h) Transwell assay conducted to study the effect of AFF4 overexpression on invasion activity of SCC1 cells and SCC23 cells. Bar indicates 200 μm. n = 3. *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001.