Table 1.

Presented studies on metabolomics and ROS.

Reference	Species	Technique	Setting	Biofluid/Tissue	Discriminate Metabolites/Metabolism
Li et al., 2015	LDLR-/- mice	NMR, GC-FID/MS	Atherosclerosis	Plasma, urine	Significant drop in PUFA-to-MUFA (and PUFA-to-UFA) ratios in both the plasma and liver of WD-fed mice
	C57BL/J6 mice			Liver, Kidney and myocardial Tissue
Chouchani et al., 2014	C57BL/6J mice	LC-MS	Ischemia-reperfusion injury	Heart	Significant increase in succinate
Lu et al., 2017	Human	QTOF/MS	Coronary Heart Disease	Plasma	glycerophospholipid metabolism including phosphatidylcholine, lysophosphatidylcholine (lysoPC), phospha- tidylethanolamine, lysophosphatidylethanolamine (lysoPE), phos- phatidylserine, lysophosphatidylserine, phosphatidylino- sitol, and lysophosphatidic acids. Among them, lysoPC (20:0), lysoPC (20:1), lysoPC (20:2), lysoPC (20:5), lysoPC (22:5), lysoPE (18:3), and glycerophosphocholine
Wang et al., 2017	Sprague-Dawley rats	UPLC-TOF/MS	Pulmonary Arterial Hypertension	Right Ventricular Tissue	Significant increase in oxidized glutathione, xanthine and uric acid and a reduction in α-tocopherol nicotinate
Varvarousis et al., 2017	Landrace/large-white pigs	NMR, LC-MS/MS	Asphyxial cardiac arrest	Plasma	Significant increase in succinate

NMR, ¹H Nuclear magnetic resonance spectroscopy; GC-FID/MS, gas chromatography with flame ionization detection – mass spectrometry; LC-MS, liquid chromatography – mass spectrometry; QTOF/MS, quadrupole time of flight mass spectrometry; UPLC-TOF/MS, ultra performance liquid chromatography – time of flight mass spectrometry; PUFA, poly-unsaturated fatty acids; MUFA, mono-unsaturated fatty acids; UFA, unsaturated fatty acids; WD, western diet; LysoPC, lysophosphatidylcholine.