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Purification and enzymatic assay of the recombinant His-tagged RcUGT1. (a) Purification of the recombinant His-tagged RcUGT1 from E. coli. (b) The HPLC traces of RcUGT1-mediated reactions: 1 represented the authentic icariside D2 and salidroside; 2 was the reaction system of control in which RcUGT1 was denatured by boiling; 3 represented the product (icariside 2) given by RcUGT1. (c) The mass spectrum of icariside D2 produced by RcUGT1.
