Table 1.
Primer | Sequence(5′-3′) | Aim |
---|---|---|
dF | GGCATCCCGCGGCTNGTNTTYCAYG | Cloning of the core sequence |
dR | CGGTCACCAGCTTCTCGTTGWARAAYTGNTC | Cloning of the core sequence |
Primer 3′-1 | GCCGGACTCGGTCGTGTACGT | 3′ RACE |
Primer 3′-2 | GAGAAGTTCCGAGTCAGGAT | 3′ RACE |
Primer 5′-1 | GACCATTTCTGCACTCCAACCT | 5′ RACE |
Primer 5′-2 | GAACAACGGCCACDTCACCAT | 5′ RACE |
pF | CGCGGATCCATGGATTCTGATTCACGGCCT | Vector construction |
pR | CGCGGTACCCTAGGACAAAGGCACTCTTCT | Vector construction |
Primer qF | ACTCATTGCGGATGGAAT | qPCR |
Primer qR | CCTTCTCAACCTTCTCCTT | qPCR |