Fig. 2.

BAL MDRCs were transduced to express CellLight Mitochondria-GFP. Mitochondria-GFP (Mito-GFP) had proper subcellular localization as seen by the MitoTracker Red (MitoT-Red) overlay. (a) Schematic for validation of transduction and subcellular localization of Mito-GFP (b) ImageStream flow cytometry images illustrating Mito-GFP expression and co-localization with MitoT-Red. (c) Quantitation of % HLA-DR⁺ MDRCs that are positive for Mito-GFP, MitoT-Red, or both (*** p < 0.001; unpaired Student's T-test) (d-e) Quantitation of mean fluorescence intensity (MFI) of either Mito-GFP or MitoT-Red signal in HLA-DR⁺ MDRCs gated for either Mito-GFP⁺, MitoT-Red⁺, or Mito-GFP⁺MitoT-Red⁺ (*** p < 0.001; unpaired Student's T-test) (n = 3 subjects, 3 technical replicates).