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. 2018 Jun 25;24:425–433.

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Copyright © 2018 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Enhanced respiratory capacity of polarized hfRPE cells compared to replated controls. A: Pictorial representation of a mitochondrial stress test and respiration parameters calculated. B: Paired wells of a single human fetal RPE (hfRPE) cell line were subjected to mitochondrial stress tests on an XFp Analyzer with two different methods. Triplicate samples of polarized cells excised from a Transwell filter (green) were compared to triplicate samples trypsinized from a Transwell filter and replated in a miniplate (black). C: Respiration parameters calculated from the mitochondrial stress test. D: Basal extracellular acidification rate (ECAR) values obtained during the mitochondrial stress test revealed a statistically significant difference between replated and insert hfRPE, and differences in the OCR/ECAR ratios (E). ****p<0.0001. Results are expressed as mean ± standard deviation.