Figure 2. DNA conjugation correlates with T6SS killing in vivo.
Competition assay was performed between donor C6706 lacZ J13 and recipient wild type C6706 or C6706 vipA tsiV3 (T6SS− and T6SS-sensitive). (A) Cells were recovered from the distal small intestine (DSI) and cecum at 3 hr post infection. Each data point indicates the C.I. relative to C6706 lacZ J13 for each experimental animal (B) Conjugation efficiency was determined for each group. (C) Donor and recipient bacterial population were above the 105 CFU/cm tissue minimum cell density thresholds. Bars indicate mean and standard error. Significance was determined by t test. * - p < 5 × 10−2, *** - p < 10−3.