Table 2.

Details of primers used in the present study

Primer	Primer Sequence	PCR product size (base pair)	Annealing Temperatu-re (°C)
Mutation primers
YAP1 exon 1 (serine 61)	F 5’-AGGCAGAAGCCATGGATC-3’	338	56.6
	R 5’-GGTTACCTGTCGGGAGTG-3’
YAP1 exon 2 (serine 109 and 127)	F 5’-GGCTGCAATTAAGCGCTGAC-3’	292	61.5
	R 5’-TGCTGGCAGAGGTACATCATC-3’
YAP1 exon 2 (serine 164)	F 5’-CGAGCTCATTCCTCTCCAGC-3’	236	55.5
	R 5’-AGATAACTGTCTCCCACC-3’
YAP1 exon 8 (serine 397)	F 5’-TTCAGACATTGCAGGACAGG-3’	248	58.8
	R 5’-CCTGTATCCATCTCATCCACAC-3’
YAP1 exon 9 (lysine 494)	F 5’-CTCTGTGTGTTTCCACTAGG-3’	317	57.5
	R 5’-CCGGTGCATGTGTCTCCTTAG-3’
Methylation primers
YAP1 methylation	F 5’-AGTTCGTATAGGCGTTTCGTTC-3’	187	57.9
	F 5’-CTTAACTACAAAAAATTCTTCCGCT-3’
YAP1 unmethylation	F 5’-AAGTTTGTATAGGTGTTTTGTTTGG-3’	188	57.9
	F 5’-CTTAACTACAAAAAATTCTTCCACT-3’
Expression primers
YAP1	F 5’-AAGCTGCCCGACTCCTTCTTCAAG-3’	161	64.1
	R 5’-GTCAGTGTCCCAGGAGAAACA-3’
GAPDH	F 5’-CACTGCCACCCAGAAGACTG-3’	150	61
	R 5’-ATGCCAGTGAGCTTCCCGTT-3’