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Sialin transports 9AzSia from the lysosome into the cytosol. FR+ HeLa cells were transfected with EGFP-sialin or the empty EGFP-C1 vector. The cells were incubated with 9AzSia (A), f-LP-9AzSia (B) or LP-9AzSia (C) at varied concentrations for 12 h. The cells were then reacted with DBCO-biotin and streptavidin-AF647, and analyzed by flow cytometry. MFI, mean fluorescence intensity. a. u., arbitrary unit. Error bars represent ± SD.
