Figure 3.

Effect of GDF15 on the NSCLC Cell Cycle and Cell Apoptosis In Vitro

(A) Forty-eight hours after transfection, the cell cycle stages of SPCA1 and H1299 cells were analyzed by flow cytometry. The bar chart represents the percentages of cells in G1/G0, S, or G2/M phases as indicated. (B) SPCA1 and H1299 cells were stained and analyzed by flow cytometry 48 hr after transfection. LR, early apoptotic cells; UR, terminal apoptotic cells. All experiments were conducted in biological triplicates with three technical replicates. Data are presented as the mean ± SD. *p < 0.05 and **p < 0.01.