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. Author manuscript; available in PMC: 2018 Oct 28.
Published in final edited form as: J Control Release. 2018 Feb 20;276:50–58. doi: 10.1016/j.jconrel.2018.02.017

Figure 9.

Figure 9

GAPDH gene knockdown of CP and VIPER in vivo. GAPDH gene knockdown efficiency was validated in whole lung RNA isolated from Balb/c mice that were instilled with melittin conjugated block copolymer (VIPER) or unmodified block copolymer (CP) at N/P 8 with 2 nmol msGAPDH siRNA. Blank samples consisted of Balb/c mice instilled with 5% glucose only. 25k polyethylenimine (PEI) polyplexes at N/P 8 with 2 nmol siRNA were instilled into Balb/c mice as a non-toxic transfection standard. GAPDH expression was normalized with β-actin expression and quantified by real time PCR. Data points indicate mean ± SD (n=6). Two-way ANOVA, *P < 0.05.