Fig. 4.
Analysis of the role of TDP-43 domains in its compartmentalization on microtubules. We analyzed compartmentalization of tau–GFP–TDP-43 or tau–GFP–G3BP1 (in green) coexisting with either TDP-43ΔRBD and TDP-43ΔLCD (in red) in HeLa cells. Left panel, fluorescence images. Right panel, analysis of compartmenting. Removing the RBD of TDP-43 causes demixing with full-length TDP-43 and mixing with G3BP1. Scale bar: 10 µm. **P<0.01 (two-tailed t-tests) for compartment enrichment data (length analyzed along the microtubule network >0.5 mm, ncell=10).