Fig. 2.

Effects of time on differentiation of THP-1 cells to macrophage-like cells induced by PMA. (A) After 24 h, the THP-1 cell induced by PMA started to adhere, cluster and very few cells presented deformation. (B) After 48 h, most of the THP-1 cells were macrophage-like, but the cells were still small. (C) After 72 h, the THP-1 cells were macrophage-like and had grown much larger. (D) After 96 h, the state of the THP-1 cells was similar to at 72 h. (A) 24 h (×100). (B) 48 h (×100). (C) 72 h (×100). (D) 96 h (×100).