Fig. 2.

mIP2-restricted AAV expression. PBS control or AAV vectors were administered via intraperitoneal injection. Mice were sacrificed for analysis 2 weeks after injection. (A) Schematics of AAV vector constructs with mIP2 driving expression of luciferase (AAV-mIP2-luciferase, top), GFP (AAV-mIP2-GFP, middle), and mouse GCK (AAV-mIP2-GCK, bottom) followed by the human growth hormone (hGH) poly-A. (B) Luminescence imaging of whole mice injected with AAV-mIP2-luciferase. Color scale represents luminescence in radiance (p/s/cm³/sr; minimum, 2×10⁵; maximum, 2×10⁶). (C) Representative fluorescent confocal microscopy images of pancreatic islets (top) with surrounding exocrine tissue (bottom) from AAV-mIP2-GFP-treated mice. Green, GFP; red, insulin; blue, DAPI. Scale bars: 50 µm. (D) Relative expression of AAV-specific Gck transcripts (GenBank BC011139.1) in pancreases of 8-month-old mice fed a chow diet (n=7-10 per group). (E) Relative expression of AAV-specific Gck transcripts (GenBank BC011139.1) in isolated islets of 8-month-old mice fed a chow diet (n=4-5 per group). (F) Relative expression of AAV-derived Gck transcripts in livers of 8-month-old mice fed a chow diet (n=5 per group). (G) Relative expression of AAV-specific Gck transcripts (GenBank BC011139.1) in pancreases of 8-month-old mice fed a HFD (n=5 per group). (H) Relative expression of AAV-specific Gck transcripts (GenBank# BC011139.1) in isolated islets of 8-month-old mice fed a HFD (n=5 per group). (I) Relative expression of AAV-derived Gck transcripts in livers of 8-month-old mice fed a HFD (n=5 per group). (J) Relative expression of total (endogenous and exogenous) Gck transcripts in livers of 8-month-old mice fed a chow diet (n=5 per group). (K) Relative total expression of total (endogenous and exogenous) Gck transcripts in livers of 8-month-old mice fed a HFD (n=5 per group). Dashed lines indicate the limit of detection. Results are shown as scatter plots with medians (black bars)±MAD.