Fig. 7.

Nmnat mitigates paclitaxel-induced sensory dysfunction. (A) Withdrawal response of ppk-Gal4 control larvae, UAS-Nmnat control and larvae overexpressing Nmnat in C4da sensory neurons (ppk-Gal4>UAS-Nmnat) after 48 h exposure to either vehicle or paclitaxel. n=50 larvae tested for each genotype and treatment, response rate: Kruskal–Wallis with Dunn's multiple comparisons test, mean withdrawal latency: one-way ANOVA with Tukey's multiple comparisons test. (B) Cumulative distribution of latency curve representative of all larvae tested in A plotted as % accumulated response as a function of withdrawal latency. Mantel–Cox test and adjusted for multiple comparisons by Bonferroni-corrected threshold, here 0.001/9=0.00011. ***P<0.00011. (C) Nmnat mRNA levels measured from whole larvae of all genotypes at 120 h AEL (ppk-Gal4 control, UAS-Nmnat control, and Nmnat overexpression in C4da sensory neurons ppk-Gal4>UAS-Nmnat) by quantitative real-time PCR expressed relative to the housekeeping gene Rp49 and normalized to ppk-Gal4 control. Mean±s.e.m.; n=4 independent tests of 5-10 larvae from each group. (D) Effect of paclitaxel treatment on growth of ppk-Gal4 control larvae, UAS-Nmnat control and larvae overexpressing Nmnat in C4da sensory neurons (ppk-Gal4>UAS-Nmnat). Larval body area was calculated as the length multiplied by the width of each larva measured in ImageJ from images acquired from a dissection microscope. Mean±s.e.m.; one-way ANOVA with Tukey's multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.