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. 2018 Jun 4;11(6):dmm032201. doi: 10.1242/dmm.032201

Fig. 1.

Fig. 1.

Generation of DMD KO rabbits using CRISPR/Cas9. (A) The common mutations in exon 51 in human DMD patients. (B) Schematic diagram of two sgRNA target sites located in exon 51 of the rabbit DMD locus. DMD exons are indicated by purple boxes; target sites of the two sgRNA sequences (sgRNA1 and sgRNA2) are highlighted in red; protospacer-adjacent motif (PAM) sequences are highlighted in green. (C,D) Mutation detection by the T7E1 cleavage assay in pups 1-16 (C) and 17-33 (D). M, the DNA ladder (DL2000).