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. 2018 Jun 25;131(12):jcs213314. doi: 10.1242/jcs.213314

Fig. 3.

Fig. 3.

Sphingolipids send nutrient transporters to the tubular recycling endosome prior to its dissolution. (A) HeLa cells treated with 893 (10 µM) for 0.5, 1, 3 or 6 h were stained for CD98. (B) HeLa cells expressing GFP-MICAL-L1 were treated with 893 (10 µM) for 1 h and stained for CD98. (C) HeLa cells treated as in A but stained for MICAL-L1. (D) Percentage of cells that exhibit tubular MICAL-L1 staining. Means±s.e.m. shown of n≥3. An ordinary one-way ANOVA was used to compare with time zero, ***P≤0.001. Dunnett's test was used to correct for multiple comparisons. (E) Representative blot for HeLa cells treated with FTY720 (10 µM), 893 (10 µM), C2-ceramide (50 µM) for 6 h, lysed, and blotted for MICAL-L1 and tubulin. (F) HeLa cells expressing GFP-MICAL-L1 treated with 893 (10 µM) for 6 h and stained for CD98. Scale bars: 10 µm.