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A phosphomimetic mutant of GRP1 protects nutrient transporter recycling from inhibition by 893. (A) HeLa cells expressing GFP alone, myc-tagged wild-type GRP1, or myc-tagged GRP1 S155D+T280D (GRP1-DD) were treated with 893 (5 µM) for 6 h and stained for GFP or myc tag and CD98. (B) As in A, except cells were stained for MICAL-L1 and myc tag or GFP. Scale bars: 10 µm. (C) Model for regulation of endocytic recycling by sphingolipids.
