Table 1.

Sequence information and diversity estimates, including singleton OTUs after rarefying to an even 2,496 sequence depth, of the Endozoicomonas community in coral and seawater samples from Kochi, as represented in V1–V2 regions of the 16S rRNA gene, detected with bacterial universal primers (U) and Endozoicomonas-specific primers (S).

Sample	Na		OTUb			Richnessc		Gini-Simpson		Shannon		Evennessd
Primer	U	S	U	S	C	U	S	U	S	U	S	U	S
Sea3S1	1	2509	1	88	0	N.D.	0.416	N.D.	0.774	N.D.	2.20	N.D.	0.491
Acr6S1	2476	2500	53	69	29	0.407	0.643	0.863	0.843	2.28	2.16	0.575	0.509
Acr5S1	2474	2502	62	54	28	0.270	0.436	0.819	0.858	2.35	2.34	0.570	0.586
Acr6D1	2490	2498	71	56	37	0.514	0.509	0.816	0.827	2.27	2.12	0.531	0.526
Acr5D1	2480	2506	71	73	38	0.361	0.486	0.879	0.844	2.53	2.31	0.593	0.539
Acr6A2	2485	2500	57	60	36	0.362	0.492	0.796	0.841	2.12	2.14	0.525	0.523
Acr5A2	2480	2496	59	47	28	0.467	0.542	0.447	0.290	1.35	0.88	0.331	0.228
Acr6S2	2491	2500	53	58	31	0.370	0.492	0.460	0.397	1.37	1.14	0.346	0.282
Acr5S2	2484	2501	49	46	29	0.320	0.340	0.377	0.398	1.15	1.16	0.295	0.304
Total	–	–	103	138	82	–	–	–	–	–	–	–	–

U=Bacterial universal primers; S=Endozocomonas-specific primers; C=Common OTUs shared between U and S data.

N.D.=undetectable in samples.

^aN defined as the number of sequences.

^bCalculations were based on operational taxonomic units (OTUs) formed at an evolutionary distance of <0.03 (or approximately 97% similarity).

There were total 159 OTUs.

^cCalculated as S/(N+1) where S is the number of singleton OTUs and N is the total number of OTUs.

^dShannon index divided by the logarithm of the number of OTUs