Table 2.

Sequence information and diversity estimates using our newly designed Endozoicomonas-specific primers in V1–V2 and V3–V4 regions of the 16S rRNA gene.

Sample	Na		OTUb		Gini-Simpson		Shannon		Evennessc
Primer	V1V2	V3V4	V1V2	V3V4	V1V2	V3V4	V1V2	V3V4	V1V2	V3V4
Mil1S1	5094	2132	21	11	0.11	0.03	0.26	0.10	0.08	0.04
Mil2S1	5626	2664	36	19	0.04	0.03	0.15	0.12	0.04	0.04
Hel1S1	8829	4336	56	27	0.10	0.04	0.31	0.14	0.08	0.04
Hel2S1	701	405	30	29	0.72	0.75	1.95	2.05	0.57	0.61
Pal1S1	4129	4917	81	47	0.85	0.72	2.43	1.56	0.55	0.41
Pal2S1	7428	4723	69	43	0.41	0.26	1.15	0.76	0.27	0.20
Sty1S1	4197	2220	32	17	0.13	0.12	0.37	0.35	0.11	0.12
Sty2S1	189	78	9	9	0.40	0.62	0.83	1.38	0.38	0.63
Fav1A2	2205	2507	63	39	0.68	0.82	1.90	2.21	0.46	0.60
Fav2A2	4298	2220	82	51	0.85	0.83	2.35	2.31	0.53	0.59
Iso1M1	5543	2663	43	22	0.23	0.03	0.59	0.11	0.16	0.04
Iso2M1	7881	4618	49	21	0.51	0.03	1.04	0.13	0.27	0.04
Acr1S1	8286	5155	33	14	0.07	0.02	0.22	0.08	0.06	0.03
Acr2S1	8092	4336	74	38	0.82	0.50	2.07	0.95	0.48	0.26
Mon1S1	4779	3055	54	35	0.42	0.55	1.16	1.27	0.29	0.36
Mon2S1	5276	3874	76	33	0.20	0.55	0.70	1.14	0.16	0.33
Eup1S1	7706	4426	64	39	0.69	0.78	1.57	1.82	0.38	0.50
Eup2A2	6630	3454	45	25	0.78	0.52	1.86	1.08	0.49	0.34
Acr3S2	2840	1415	38	25	0.65	0.66	1.73	1.43	0.48	0.44
Acr4S2	824	207	20	10	0.64	0.69	1.36	1.46	0.45	0.64
Eup3S2	1896	325	62	25	0.47	0.64	1.36	1.76	0.33	0.55
Eup4S2	1964	725	15	9	0.24	0.49	0.62	0.77	0.23	0.35
Acr5S1	2435	565	54	14	0.88	0.57	2.61	1.30	0.65	0.49
Acr6S1	3091	654	46	16	0.84	0.40	2.14	0.96	0.56	0.35
Acr5D1	2890	725	59	16	0.84	0.20	2.31	0.56	0.57	0.20
Acr6D1	2749	570	44	13	0.83	0.53	2.13	1.06	0.56	0.41
Acr5A2	2473	1585	43	16	0.39	0.38	0.83	0.88	0.22	0.32
Acr6A2	2609	455	45	11	0.84	0.41	2.11	0.90	0.56	0.37
Acr5S2	2705	419	40	8	0.46	0.51	1.13	1.10	0.31	0.53
Acr6S2	3090	527	38	12	0.52	0.48	1.21	1.05	0.33	0.42
Sea1S1	1288	860	98	71	0.94	0.94	3.27	3.24	0.71	0.76
Sea2S2	666	451	60	61	0.94	0.93	3.20	3.31	0.78	0.81
Sea3S1	2909	1916	88	73	0.81	0.88	2.34	2.77	0.52	0.65

The highest values in each column are in bold.

^aN defined as the number of sequences.

^bOperational taxonomic units (OTUs) formed at an evolutionary distance of <0.03 (or approximately97% similarity).

^cShannon index divided by the logarithm of the number of OTUs