Fig. 2.

Identification of Hnf1b and Fzd4 as direct endodermal RA target genes. (A) Experimental procedure for the identification of early direct RA target genes in the context of pancreas development. (B) Venn diagram comparing genes differentially expressed within 2 h of RA addition in the absence or presence of CHX. A total of 46 putative direct RA target genes were induced under both conditions. (C) RNA-seq results for RA-mediated Hnf1b and Fzd4 induction. The number of mapped reads 2 h after the addition of RA in the presence or absence of CHX is indicated. The data result from two biological replicates with ∼50 explants per condition. Average values are given as mean and error bars as s.e.m. (D) WMISH for Hnf1b and Fzd4 in gastrula-stage embryos. Whole embryos are depicted on the left-hand side and bisected embryos on the right-hand side. dbl, dorsal blastopore lip; e, endoderm; m, mesoderm; ne, neuroectoderm.