Fig. 2.

Test tube validation of split-initiator HCR suppression. (A) Agarose gel electrophoresis. Reaction conditions: hairpins H1 and H2 at 0.5 µM each (lanes 1-7); initiator I1, probes P1 and P2 (each carrying half of initiator I1; Fig. 1B), and/or DNA target at 5 nM each (lanes noted on the gel); 5× SSCT buffer; overnight reaction at room temperature. Hairpins H1 and H2 were labeled with Alexa 647 fluorophore (green channel); a dsDNA 1 kb ladder was pre-stained with SYBR Gold (red channel). (B) Quantification of polymer bands in A. See Figs S3 and S4 for additional data.