Table 1. Dissociation constants for PRPP and ppGpp binding to the wild type and G96A T. mathranii aptamers with calculated fold specificity changes.

Dissociation constants for WT and G96A binding to PRPP and ppGpp
Construct	Kd for PRPP	Kd for ppGpp	Fold specificity for PRPP over ppGpp	Estimated magnitude of overall specificity switch
Wild type	2.0 ± 0.3 μM	91 ± 3 μM	46	~40,000
G96A	1600 ± 200 μM	1.8 ± 0.1 μM	~0.001