Fig. 1. Assessment of pSer129-α-Syn and inclusion detection in mouse brain.

Wild-type and α-syn knockout mice (C57bl/6J) were injected bilaterally into the striatum with 10 µg of α-syn pre-formed fibrils or monomeric protein as previously described(Abdelmotilib et al., 2017). Brain sections were evaluated from mice six months later. Representative widefield images of DAB-staining (converted to grayscale for contrast) with the indicated monoclonal antibody (left margins) in animals injected with A) fibrils in wild-type mice, B) fibrils in α-Syn knockout mice, or C) monomer protein in wild-type mice. All antibodies were included at 10 ng mL⁻¹ concentration. Coronal sections are shown with 0.4 mm of Bregma, with primary motor cortex (ctx) and dorsal striatum (str) shown at two different magnifications. Scale bars are 0.1 mm.