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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: J Comp Neurol. 2018 Aug 15;526(12):1978–1990. doi: 10.1002/cne.24468

Fig 6. Confocal analysis of pS129 α-syn-positive inclusions in mice and rats.

Fig 6

C57Bl/6J wild-type mice and Sprague Dawley wild-type rats were injected bilaterally into the striatum with either 10 µg or 20 µg (respectively) of α-syn fibrils and frozen brain sections analyzed six months later. Confocal images were obtained from a single plane with a 63× objective set for optimal pinhole. Red color indicates pS129-α-syn staining (Cy3) and blue color indicates DAPI. The monoclonal antibody used at 20 ng mL−1 are given in the left margin. Images from both the primary motor cortex (cortex) and dorsal striatum (striatum) were obtained. Scale bar is 50 µm.