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. 2018 Jul 4;8:10130. doi: 10.1038/s41598-018-28497-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in mitochondrial function and dynamics in the G1 and G2 groups. (a) Expression of J-aggregate (red) and J-monomer (green) were determined by JC-1 using a confocal microscope. (b) ATP contents were measured by an ATP determination kit using a microplate reader. (c) Mitotracker (green) as the mitochondria-selective probe was measured under image system. N indicates the nucleus and arrows represent mitochondrial aggregation form. (d) Cropped blots are used in this figure, and the gels have been run under the same experimental conditions. Full-length blots are included for key data in the supplementary information. Western blot showing expression of mitochondrial fission proteins (Drp1 and phosphorylated Drp1-Ser616). Reactive protein levels were normalized to β-actin. Data in the bar graph represent means ± SEM from three independent experiments. To analyze these data, Dunnett’s multiple comparison test and t-test were used. Differences were considered significant at *p < 0.05 and ** < 0.01. Scale bar = 50 μm.