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. 2018 Jul 4;8:10130. doi: 10.1038/s41598-018-28497-5

Figure 4.

Figure 4

Effects of Mito-TEMPO on early embryo development in porcine. (a) Red stars indicate the number of expanded blastocysts. Morphologies of blastocyst stage in G1, G2 and G2 with Mito-TEMPO treatment. Detection of (b) superoxide (red fluorescence) and (c) mitochondrial distribution (green fluorescence) in G2 zygotes treated with Mito-TEMPO during embryo development. The arrows show aggregation of mitochondria and N represents the nucleus. Data in the bar graph represent the means ± SEM from three independent experiments. To analyze these data, Tukey’s multiple comparison test was used. Differences were considered significant at *p < 0.05 and ** < 0.01. Scale bar = 50 μm.