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. 2018 Jul 4;8:10130. doi: 10.1038/s41598-018-28497-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagram illustrating the effect of Mito-TEMPO on porcine embryo development. Top panel; zygotes with low cytoplasm had increasing mitochondrial superoxide. In addition, high superoxide production in G2 embryos caused defective MMP and ATP production. Mitochondrial fission and aggregation increased in G2 embryos. Bottom panel; Zygotes of low cytoplasm cultured with Mito-TEMPO for early embryo development. Reduction of mitochondrial superoxide increases the blastocysts development rate and decreases mitochondrial aggregation in porcine.