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. 2018 Jul 4;8:10082. doi: 10.1038/s41598-018-28414-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the polysaccharide formula on lymphocytes. (A and B) Proportion and activation of T and B cells. Lymphocytes isolated from the spleen were stained with CD3-PEcy7, CD19-PE and CD69-FITC, and analyzed by flow cytometry. CD3⁺ (P4) were considered T cells, CD19⁺ (P3) were considered B cells, CD3⁺CD69⁺ (Q2) were considered activated T cells, and CD19⁺CD69⁺ were considered activated B cells (Q2-1). (C) Cytokines in peripheral blood, *P < 0.05. (D) Antibody in serum and the small intestine, *p < 0.05.