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. 2018 Jul 4;8:10078. doi: 10.1038/s41598-018-28441-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expanded 2D plots (A) and (C) of ¹H-¹⁵N HSQC TROSY type spectra of the complex ⁵N/¹³C labelled NS3-NS2Bpro with Bz-Nle-Lys-Arg-Arg-B(OH)₂, showing one bond ¹H-¹⁵N correlation with corresponding 1D ¹H spectrum presented on top (B) and (D) of the 2D spectra. In expansion (A) amide cross peak of amino acid K84 at 11.396/127.85ppm is shown as reference. Cross peak at 15.587/178.7ppm is assigned to the N^ε2H of His51. In expansion (C) cross peak at 19.772/207.6ppm is assigned to the N^δ1H of His51 of the other form.