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. 2018 Jul 4;8:10134. doi: 10.1038/s41598-018-27740-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Luciferase assay validation for selected miRNAs and Adrb2 mRNA 3′UTR interaction. Luciferase signal intensity was significantly downregulated by selected targeting miRNAs, also miR-106 and miR-322, compared to non-targeting control miRNA (A). *<0.05 vs. non-targeting control, n = 3 (one-way ANOVA followed by Dunnett’s post hoc test) Representative maps of Lightswitch luciferase reporter vector (B) and beta-galactosidase reporter vector (C) used for miRNA luciferase assay.