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. 2018 Jul 4;8:10097. doi: 10.1038/s41598-018-28179-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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msp1, msp2 HRM discrimination of artificial mixes of parasite clones at different ratios. Normalized temperature-shifted HRM amplification curves for msp1 (a1,c1 and e1) and msp2 (b1 and d1) and their corresponding melt curves (a2,b2,c2,d2 and e2) are shown for Dd2, K1 and 7G8 laboratory lines, and mixes of two strains at the ratios shown.