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. 2018 Jul 4;8:10097. doi: 10.1038/s41598-018-28179-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Benefits of msp1 and msp2 genotyping by real-time PCR and HRM. Summary of significant gains in simplicity, speed and interpretation of results from utilizing the qPCR-HRM genotyping approach compared to the conventional msp1 and msp2 genotyping using gel electrophoresis. *3–4 hours per run (72 samples for rotor-gene and 96 samples for other platforms).