Table 1.
Mean melting peak and mean GCP* of single and mixed laboratory strains.
| Laboratory strain | Mix ratio | Genotype called by the software | Msp1 | Msp2 | ||
|---|---|---|---|---|---|---|
| Mean GCP * | Tm* | Mean GCP* | Tm* | |||
| 3D7 | — | 3D7 | 99.3 | 86.0 | 99.9 | 84.0 and 86.5 |
| Dd2 | — | Dd2 | 97.5 | 86.4 | 99.7 | 83.50 |
| 7G8 | — | 7G8 | 99.7 | 83.3 | 99.7 | 84.3 and 87.3 |
| K1 | — | K1 | 99.9 | 84.0 and 84.3 | 99.7 | 82.8 and 83.8 |
| R033 | — | R033 | 99.9 | 82.9 | 99.5 | 84.3 and 87.3 |
| Dd2:7G8 | 1:1 | Dd2/7G8 | 99.6 | 83.2 and 86.5 | 98.7 | 83.9 and 86.9 |
| 10:1 | Dd2/7G8 | 91.8 | 83.2 and 86.5 | 74.4 | 83.9 and 86.9 | |
| 25:1 | Dd2/7G8 | 88.3 | 83.2 and 86.5 | 68.4 | 83.9 and 86.9 | |
| 50:1 | Dd2/7G8 | 76.5 | 83.3 and 86.6 | 62.7 | 83.9 and 86.9 | |
| 100:1 | Dd2/7G8 | 73.9 | 83.2 and 86.5 | 62.4 | 83.9 and 86.9 | |
| 500:1 | Dd2/7G8 | 63.15 | 83.2 and 86.5 | 59.5 | 83.9 and 86.9 | |
| 1000:1 | Dd2/7G8 | 61.4 | 83.2 and 86.5 | 52.88 | 83.9 and 86.9 | |
| 1:1000 | Dd2/7G8 | 99.3 | 83.2 and 86.3 | 56.3 | 81.9 and 84.9 | |
| 1:500 | Dd2/7G8 | 62.8 | 83.2 and 86.3 | 61.2 | 81.9 and 84.9 | |
| 1:100 | Dd2/7G8 | 73.2 | 83.2 and 86.3 | 64.5 | 81.9 and 84.9 | |
| 1:50 | Dd2/7G8 | 78.2 | 83.3 and 86.3 | 70.2 | 81.9 and 84.9 | |
| 1:25 | Dd2/7G8 | 89.2 | 83.2 and 86.3 | 73.6 | 81.9 and 84.9 | |
| 1:10 | Dd2/7G8 | 91.2 | 83.2 and 86.3 | 78.5 | 81.9 and 84.9 | |
| 1:1 | Dd2/7G8 | 99.3 | 83.2 and 86.3 | 97.9 | 81.9 and 84.9 | |
| 1:1 | Dd2/K1 | 99.8 | 84.0 and 86.0 | — | — | |
| 10:1 | Dd2/K1 | 93.9 | 84.0 and 86.0 | — | — | |
| 25:1 | Dd2/K1 | 89.7 | 84.0 and 86.0 | — | — | |
| 50:1 | Dd2/K1 | 86.5 | 84.0 and 86.0 | — | — | |
| 100:1 | Dd2/K1 | 83.5 | 84.0 and 86.0 | — | — | |
| 500:1 | Dd2/K1 | 73.2 | 84.0 and 86.0 | — | — | |
| 1000:1 | Dd2/K1 | 71.4 | 84.0 and 86.0 | — | — | |
*For samples with a single allele, the mean GCP estimates agreement between duplicate tests while the mean GCP for the mixtures is estimated from 2 mixtures of Dd2 and 7G8 at the ratio shown, relative to the 1:1 mixture.
**Tm: melting temperature; this is estimated by the rotor-gene 6000 analysis software (version 1.7). Mixture clones of Dd2 and K1 was not done as both belong to the same allelic family.