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. 2018 Jul 4;8:10137. doi: 10.1038/s41598-018-28472-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photo-physical properties of mNeonGreen and Dronpa. Histogram of detected photon number for (a) mNeonGreen or Dronpa fused to DivIVA expressing B. subtilis strains (BHF039) or Dronpa (BHF057), respectively. Mean and median of the number of photons are indicated. (b, left) Initial linear section of the relative cumulative on-switching probability vs time was fitted (red dotted line) linearly to obtain the on-switching rate. (b, right) Distribution of the on-state lifetime was fitted to an exponential decay function (red dotted line) to obtain the mean life time, the inverse of which yields the off-switching rate. One frame corresponds to 20 ms. (See also Supplementary Table I).