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. 2018 Jul 4;9:2607. doi: 10.1038/s41467-018-05061-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Transport of NPs with various rigidities in rat mucus ex vivo. a Representative trajectories of particle motion in 1 s in fresh rat intestinal mucus. b <MSD> values as a function of time for particles in mucus. c Distributions of the logarithms of individual particle effective diffusivity (D_eff) values in mucus on a time scale of 1 s. The data represent three independent experiments, with each experiment tracking 100 NPs. ****P < 0.0001, PLGA₇₀-Lip-F127_5% compared to Lip-F127_5%, and ^####P < 0.0001, PLGA₇₀-Lip-F127_5% compared to PLGA₁₆₀-Lip-F127_5%