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. 2018 Jul 4;8:10126. doi: 10.1038/s41598-018-28293-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative AFM/KFM images of collagen fibrils. Topography (a and c) and surface potential (b and d) of randomly selected collagen fibrils on glass. Top row (a and b) sample exposed to GA-solution; bottom row (c and d) sample exposed to control solution without GA. Images taken >48 h after removal of the sample from the solutions and drying in ambient conditions (ca. 40–50% relative humidity). Below the images: Longitudinal topography profiles as indicated in the images (a and c), cross-sectional potential profiles as indicated in the images (b and d). The white, dashed rectangle in (b) shows an example of the approximate area which was used for determining the fibril potential in the image analysis avoiding the “edge effect”.