Figure 2.

First, second, and ninth generation females maintain four distinct gut microbiota (GM) communities similar to surrogate dams. Principal coordinate analysis (PCoA) of representative fecal samples from surrogate dams (n = 5) and 6 to 8 week-old CD1 female offspring, first and second generation (n = 8–10) and ninth generation (n = 18–20). Figure legend of GM and generational groups located right of PCoA. Statistical significance determined using two-way PERMANOVA (p ≤ 0.05 statistically significant).