Fig. 7.

Impact of natural variants on IRES-mediated translation.a Sequence of the G28A mutant compared to wild type. b Translation assay of WT versus G28A in the presence of miR^mut or miR^WT. c Replication assay of WT versus G28A in presence of miR^mut or miR^WT. d Representation of the predicted structural ensemble for the first 120 nt of the WT, as predicted by RNA structure and their respective free energy values (−ΔG [kcal/mol]). e As in (d) for the G28A mutant. Mean values (±SD), n = 3, in technical duplicates. RLU relative light units, ΔGDD replication deficient mutant. For the translation assay, statistical significance of the difference between WT and G28A mutants in presence of miR^mut is indicated. The reference graph used to calculate is given in light gray. *P < 0.05, **P < 0.01, ***P < 0.001