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. 2018 Jun 28;9:648. doi: 10.3389/fphar.2018.00648

FIGURE 7.

FIGURE 7

GW501516 inhibited gene and protein expression of integrin-linked kinase (ILK) in C666-1 cells. (A) ILK mRNA expression in C666-1 cells. Cells were exposed to indicate concentrations of GW501516 (GW) for 24 h and then gene expression of ILK were analyzed by QPCR. Gene expression results are normalized to β-actin in the correspondent treatment group, and then expressed as relative expression compared with that in the vehicle group. (B) ILK protein expression in C666-1 cells after treatment with increasing concentration of GW501516 for 24 h (upper panel), or treated with 30 μM GW501516 for the indicated time (lower panel). (C,D) AMPK inhibitor compound C (Compd.C) abrogated the suppressing effect of GW501516 on ILK gene and protein expression in C666-1 cells. Cells were treated with compound C (20 μM) for 30 min before exposure to GW501516 (30 μM) for an additional 24 h. After the incubation, RNA and protein were extracted from cells for QPCR and western blot assays, respectively. GAPDH was used as loading control in the western blot. n = 3, values are mean ± SD. ∗P < 0.05, ∗∗P < 0.01 versus the control (Con.) group. #P < 0.05, ##P < 0.01 versus the GW group.