Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 28;6:87. doi: 10.3389/fbioe.2018.00087

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Vasilevich, Mourcin, Mentink, Hulshof, Beijer, Zhao, Levers, Papenburg, Singh, Carpenter, Stamatialis, Blitterswijk, Tarte and Boer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Characterization of TSCs in comparison with BM-MSCs. (A) BM-MSCs and TSCs were screened on a panel of mesenchymal markers. (B,C) TSCs were cultured with and without TNF-α and LT-α1β2 for 48 h. ICAM-1 expression was assessed by FACS analysis (B), and by immunofluorescent staining (C). (D) BM-MSCs and TSCs were cultured in adipogenic medium for 3 weeks; lipids were stained with oil red O. (E) Lipid quantification was performed by lysis of stained lipids with Oil red O in a mixture of isopropanol and Igepal. (F) BM-MSCs and TSCs were cultured in osteogenic medium for 4 weeks; deposited calcium was stained with Alizarin red. (G) Calcium deposition was quantified using a calcium assay kit. *Indicates significant difference with a p < 0.05. ****Indicates significant difference with a p < 0.0001.